The synthetic peptide SVVYGLR promotes myogenic cell motility <i>via</i> the TGFβ1/Smad signaling pathway and facilitates skeletal myogenic differentiation <i>in vitro </i>

In the present study, we investigated possible involvement of TGF-β/Smad signaling pathway in osteopontin-derived SVVYGLR (SV) peptide-mediated migratory activities myogenic cells and evaluated facilitative effects SV peptide on differentiation vitro. The peptide-induced migration both human-derived satellite myoblasts was substantially suppressed by TGF-β1 receptor inhibitor SB431542 or SB505124. Besides, expression level Smad3 phosphorylation further enhanced addition comparison with control groups. Furthermore, an increase myogenin-positive nuclei a higher number nascent myotubes myosin heavy chain confirmed cultured supplemented peptide. These results suggest that effect into myotubes.